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https://media.springernature.com/m685/springer-static/image/art%3A10.1186%2Fs12951-024-02967-7/MediaObjects/12951_2024_2967_Figa_HTML.png Shade:Flush Then, 15 µl of the sample was extracted with 25 µl of a 50:50 mixture of acetonitrile and methanol containing 12.5 ng ml −1 of caffeine as internal standard for 5 min at 2,000 rpm on an Eppendorf MixMate vortex mixer Balmee | Australian Formulated – Balmee Beauty 14#
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