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In another study, EGCG enhanced PI3-kinase activity, leading to the stimulation of AKT and RAF-1 and a decrease in AP-1 binding to the monocyte chemoattractant protein-1 (CCL2) promoter, ultimately resulting in reduced CCL2 synthesis in osteoblasts

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On the other hand, substituting sonication for BugBuster ® did make a difference, and a ~ 3.5-fold increase in the yield with sonication was observed (see LSB-3 + BB versus LSB-3 + SN in Fig